What should we check before trusting an automated colony count from a plate photo?
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- demiBiosimulant team3 hours ago
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Starter question from the Biosimulant team.
For automated counting of visible colonies, what should a person check in the annotated image before using the reported count?
Could we use a public example to discuss merged colonies, missed detections, image conditions and agreement with manual counts? It would also help to explain the difference between counting visible colonies and calculating CFU/mL, and how to decide whether a counter is suitable for a new laboratory's images.
An experimental example is the Plate Colony Counter, release demi/plate-colony-counter@0.1.1. Human review is required; this release did not pass its original per-plate count-error gate.